Digital MicroscopyGreenfield Sluder, David E. Wolf Elsevier, 26.04.2007 - 632 Seiten The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.
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Inhalt
| 1 | |
| 11 | |
| 43 | |
| 55 | |
Fundamentals of Fluorescence and Fluorescence Microscopy | 63 |
Fluorescent Protein Applications in Microscopy | 93 |
LiveCell Fluorescence Imaging | 115 |
Working with Classic Video | 141 |
HighResolution VideoEnhanced Differential Interference Contrast Light Microscopy | 335 |
Quantitative Analysis of Digital Microscope Images | 365 |
Evaluating Optical Aberration Using Fluorescent Microspheres Methods Analysis and Corrective Actions | 397 |
Ratio Imaging Practical Considerations for Measuring Intracellular Ca2+ and pH in Living Cells | 415 |
Computational Restoration of Fluorescence Images Noise Reduction Deconvolution and Pattern Recognition | 435 |
Quantitative Fluorescence Microscopy and Image Deconvolution | 447 |
Practical Aspects of Quantitative Confocal Microscopy | 467 |
Theoretical Principles and Practical Considerations for Fluorescence Resonance Energy Transfer Microscopy | 479 |
Practical Aspects of Adjusting Digital Cameras | 159 |
Cameras for Digital Microscopy | 171 |
A HighResolution Multimode Digital Microscope System | 187 |
Electronic Cameras for LowLight Microscopy | 219 |
Camera Technologies for Low Light Imaging Overview and Relative Advantages | 251 |
Digital Manipulation of Brightfield and Fluorescence Images Noise Reduction Contrast Enhancement and Feature Extraction | 285 |
Digital Image Files in Light Microscopy | 315 |
Fluorescence Lifetime Imaging Microscopy | 495 |
Fluorescence Correlation Spectroscopy Molecular Complexing in Solution and in Living Cells | 525 |
Breaking the Resolution Limit in Light Microscopy | 561 |
| 581 | |
Volumes In Series | 601 |
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aberration acceptor Airy disk aperture applications bright camera noise camera output CCD camera CCD chip Cell Biology chapter color condenser Confocal Microscopy contrast cooled CCD coverslip deconvolution detection detector devices diaphragm digital image diVerent diVraction diVusion donor dynamic range electrons emission energy transfer eVect excitation exposure field filter FLIM fluorescence correlation spectroscopy fluorescence imaging fluorescence lifetime fluorescence microscopy fluorescent protein fluorophore focal plane focus Fourier frame rate frequency FRET function gain gray levels gray values green fluorescent protein ICCD illumination image plane increase Inoue intensifier laser lenses lifetime imaging light intensity light level limit live-cell living cells low light magnification measurements microscope microtubules molecular molecules monitor multiple objective lens optical photobleaching photocathode photon pixel polarization quantitative quantum eYciency readout noise sample scan sensitivity shot noise signal signal-to-noise ratio specimen tion VE-DIC voltage volume wavelengths