Digital Microscopy

Cover
Greenfield Sluder, David E. Wolf
Elsevier, 26.04.2007 - 632 Seiten
The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution.
  • Expands coverage to include discussion of confocal microscopy not found in the previous edition
  • Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods
 

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Inhalt

Microscope Basics
1
The Optics of Microscope Image Formation
11
Proper Alignment of the Microscope
43
Mating Cameras to Microscopes
55
Fundamentals of Fluorescence and Fluorescence Microscopy
63
Fluorescent Protein Applications in Microscopy
93
LiveCell Fluorescence Imaging
115
Working with Classic Video
141
HighResolution VideoEnhanced Differential Interference Contrast Light Microscopy
335
Quantitative Analysis of Digital Microscope Images
365
Evaluating Optical Aberration Using Fluorescent Microspheres Methods Analysis and Corrective Actions
397
Ratio Imaging Practical Considerations for Measuring Intracellular Ca2+ and pH in Living Cells
415
Computational Restoration of Fluorescence Images Noise Reduction Deconvolution and Pattern Recognition
435
Quantitative Fluorescence Microscopy and Image Deconvolution
447
Practical Aspects of Quantitative Confocal Microscopy
467
Theoretical Principles and Practical Considerations for Fluorescence Resonance Energy Transfer Microscopy
479

Practical Aspects of Adjusting Digital Cameras
159
Cameras for Digital Microscopy
171
A HighResolution Multimode Digital Microscope System
187
Electronic Cameras for LowLight Microscopy
219
Camera Technologies for Low Light Imaging Overview and Relative Advantages
251
Digital Manipulation of Brightfield and Fluorescence Images Noise Reduction Contrast Enhancement and Feature Extraction
285
Digital Image Files in Light Microscopy
315
Fluorescence Lifetime Imaging Microscopy
495
Fluorescence Correlation Spectroscopy Molecular Complexing in Solution and in Living Cells
525
Breaking the Resolution Limit in Light Microscopy
561
Index
581
Volumes In Series
601
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