Molecular Cloning: A Laboratory Manual

V.1: Plasmids and their usefulness in molecular cloning; Bacteriophage and its vectors; Working with bacteriophage M13 vectors; Working with high-capacity vectors; Gel electrophoresis of DNA and pulsed-field agarose gel electrophoresis; Preparation and analysis of eukaryotic genomic DNA; Extraction, purification, and analysis of mRNA from eukaryotic cells; v.2: In vitro amplification of DNA by the polymerase chain reaction; Preparation of radiolabeled DNA and RNA probes; Working with synthetic oligonucleotide probes; Preparation of cDNA libraries and gene identification; DNA sequencing; Mutagenesis; Screening expression libraries; v.3: Expression of cloned genes in escherichia coli; Introducing cloned genes into cultured mammalian cells; Analysis of gene expression in cultured mammalian cells; Protein interaction technologies.